Fig 1: Schematic illustration of main findings. We proposed that deactivation of MIF by Oba prevents macrophage-derived inflammatory infiltration into prostate, which further alleviates EAP-induced central sensitization and CPPS.
Fig 2: The effect of Oba and MIF inhibitor ISO-1 on CPPS. (A) The protein level of MIF in (A) serum and (B) prostate of EAP mice. N = 4/group. Unpaired t-test. (C) The prostatodynia was assayed 7 days after MIF inhibitor application (Sham + vehicle group vs. EAP + vehicle group, ***p < 0.001 at 1 g and 4 g; EAP + vehicle group vs. EAP + ISO-1 [M] group, #p < 0.05 at 1 g). (D) The effect of Oba [M] on prostatodynia in the presence or absent of ISO-1 [M] (EAP + vehicle vs. EAP + ISO-1 [M], #p < 0.05 at 1 g; EAP + vehicle vs. EAP + Oba [M], *p < 0.05 at 1 g, **p < 0.01 at 4 g). N = 8–10/group. Two-way ANOVA followed by Bonferroni multiple comparisons test. All the values are shown as means ± SEM. Significant difference was considered when P < 0.05.
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